repository of small ORFs identified by ribosome profiling datasets. This section provides detailed information about the datasets used. For more information about the dataset processing please consult the INFO page. navigate through the datasets on the left to acquire more information about the corresponding dataset.


S2 Drosophila melanogaster cell line S2 (Schneider 2) cells were derived from a primary culture of late stage (20–24 hours old) Drosophila melanogaster embryos References Extensive translation of small Open Reading Frames revealed by Poly-Ribo-Seq.
Authors Aspden JL, Eyre-Walker YC, Phillips RJ, Amin U, Mumtaz MA, Brocard M, Couso JP.
GEO accession GSE60384
treatment type pre-lysis cycloheximide treatment nuclease used: MNase
total number of reads (CHX/EM sample) reads mapped to rRNA (CHX/EM sample) reads mapped to tRNA (CHX/EM sample) reads mapped to snRNA/snoRNA (CHX/EM sample) reads mapped to genomix(% total number of reads)
347408013 290940835 17924660 552786 21327857 (6.14%)
FastQC quality control CHX/EM data (no rRNA/tRNA/snRNA/snoRNA) FastQC provides quality control checks on raw sequence data coming from high throughput sequencing pipelines. FastQC provides a modular set of analyses which can be used to give a quick impression on the quality and potential problems of the data. Prior to the FastQC analysis, rRNA, tRNA, snRNA & snoRNA were removed from the data.

FastQC quality control CHX/EM