SORFS.org
A repository of small ORFs identified by ribosome profiling
sORFs.org datasets. This section provides detailed information about the datasets used. For more information about the dataset processing please consult the INFO page. navigate through the datasets on the left to acquire more information about the corresponding dataset.
| HEK293 | Human embryonic kidney cells 293, also often referred to as HEK 293, HEK-293, 293 cells, or less precisely as HEK cells, are a specific cell line originally derived from human embryonic kidney cells grown in tissue culture | |||
| References | The ribosome profiling strategy for monitoring translation in vivo by deep sequencing of ribosome-protected mRNA fragments. | |||
| Authors | Yoon JH, De S, Srikantan S, Abdelmohsen K, Grammatikakis I, Kim J, Kim KM, Noh JH, White EJ, Martindale JL, Yang X, Kang MJ, Wood WH 3rd, Noren Hooten N, Evans MK, Becker KG, Tripathi V, Prasanth KV, Wilson GM, Tuschl T, Ingolia NT, Hafner M, Gorospe M. | |||
| GEO accession | GSE37744 | treatment type | pre-lysis cycloheximide treatment | nuclease used: | RNase I |
| total number of reads (CHX/EM sample) | reads mapped to rRNA (CHX/EM sample) | reads mapped to tRNA (CHX/EM sample) | reads mapped to snRNA/snoRNA (CHX/EM sample) | reads mapped to genomix(% total number of reads) |
| 190371072 | 173593272 | 147576 | 753565 | 13900767 (7.30%) |
FastQC quality control CHX/EM data (no rRNA/tRNA/snRNA/snoRNA)
FastQC provides quality control checks on raw sequence data coming from high throughput sequencing pipelines.
FastQC provides a modular set of analyses which can be used to give a quick impression on the quality and potential problems of the data.
Prior to the FastQC analysis, rRNA, tRNA, snRNA & snoRNA were removed from the data.
source: http://www.bioinformatics.babraham.ac.uk/projects/fastqc/ |
FastQC quality control CHX/EM | |||