FastQCFastQC Report
Fri 7 Jul 2017
fastq1_norrna_nosnrna_notrna.fq

Summary

[OK]Basic Statistics

MeasureValue
Filenamefastq1_norrna_nosnrna_notrna.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47447147
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCGGTCCGGATCGTATGGGTTATCAATTTGGAATTCTCGGGTGCCAAGG1774880.37407517885111197Illumina Small RNA Adapter 2 (100% over 21bp)
AAGCGGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCA1107540.23342604772421827RNA PCR Primer, Index 7 (100% over 40bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA880660.1856086310099952RNA PCR Primer, Index 7 (100% over 35bp)
TCTATTAATTAATCTGACGTGATATTAGTGGAATTCTCGGGTGCCAAGGA802150.169061798383789RNA PCR Primer, Index 1 (100% over 22bp)
GAATTCCACCACGTTCCCGTGGCTGTGGTCGTGACTTCTAATTTGCTGGT781430.1646948340223702No Hit
AAGCGGAGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATC761610.1605175544063798RNA PCR Primer, Index 7 (100% over 39bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATGC661850.1394920541797803RNA PCR Primer, Index 7 (100% over 50bp)
GAATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCA629190.13260860552901105RNA PCR Primer, Index 1 (100% over 28bp)
CACCGGTCCGGAGTTTGTGGGTTACCAAAATGGAATTCTCGGGTGCCAAG511070.10771353649567171No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTAGT125250.039.1889623
TAATTAA130850.038.6106346
TTAATTA132700.038.0854385
GTCGCGC71250.038.07326523
GCGGTCC311750.037.9096342
TCTATTA133650.037.888511
CTATTAA134450.037.3425062
ATATTAG132750.037.0762122
TGCGGTC320450.037.0642281
TATTAAT136150.036.682333
TTAATCT140700.036.5504729
AATTCCA719000.036.3581472
ATAGGTC61200.035.7179681
GTATGGG336750.035.545914
GGGTTAT426600.035.41136618
TTCCACC733750.035.3681534
CTTGGTC570300.035.284911
TAGGTCC64300.035.2277262
CGGATCG336250.035.1667868
TTGGTCA483150.035.1120342
Produced by FastQC (version 0.11.2)