FastQCFastQC Report
Mon 3 Apr 2017
fastq1_norrna_nosnrna_notrna.fq

Summary

[OK]Basic Statistics

MeasureValue
Filenamefastq1_norrna_nosnrna_notrna.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38385873
Sequences flagged as poor quality0
Sequence length26-50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGTGGTTCAGTGGTAGAATTCTCGC501989013.077441276377902No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGC22956835.980541330921405No Hit
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGGC8081182.105248459504881No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC7887512.0547950023176496No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGC6986661.8201123105888461No Hit
AGAGTGGCGCAGCGGAAGCGTGCTGGGC6013321.566545067243879No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGC5002391.303185158769217No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAC4793561.2487823319792675No Hit
GTTTCCGTAGTGTAGTGGTCATCACGCTC3565880.9289563376609932No Hit
AGAGCGGCAAGAGTGAGATTTCTGAGC3527570.9189761035264197No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC3141440.8183844092851555No Hit
GCATTGTGGTTCAATGGTAGAATTCTCGC3111780.8106576083341911No Hit
AGCAGAGTCGCGCAGCGGAAGCGTGCTGGGC3097680.8069843819886551No Hit
GCATTGGTGGTTCAATGGTAGAATTCTCGC2692350.7013908476172992No Hit
GCATTGGTAGTTCAATGGTAGAATTCTCGCC2497320.6505830934208531No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCG2402100.6257770925256799No Hit
GCATTGGTAGTTCAATGGTAGAATTCTCGC2401980.6257458310248669No Hit
AGCAGAGTGCGCAGCGGAAGCGTGCTGGGC2361870.6152966743780973No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCC2354670.613420984329313No Hit
AGCAGAGTAGCGCAGCGGAAGCGTGCTGGGC2271020.5916291131375336No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGG2177280.5672086707523885No Hit
GCATGTGGTTCAGTGGTAGAATTCTCGC2129150.5546702038012786No Hit
AGAGTTGCGCAGCGGAAGCGTGCTGGGC2107300.5489780055282317No Hit
GAGTGGCGCAGCGGAAGCGTGCTGGGC2090710.5446561030408245No Hit
GCATGTGGTTCAATGGTAGAATTCTCGC1868590.48679106503582714No Hit
TCCCACATGGTCTAGCGGTTAGGATT1864540.48573598938338586No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACT1696720.442016780496304No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTC1652720.43055423019817735No Hit
GCATTGTGGTTCAATGGTAGAATTCTCGCC1627130.4238877151497896No Hit
GTTTCCGTAGTGTAGTGGTCATCACGCTCG1600280.4168929543428646No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTG1595370.4156138379345964No Hit
TCCCTGGTGGTCTAGTGGTTAGGATT1505640.39223805070162143No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGC1468520.38256782645011095No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG1362190.35486753160466095No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC1339300.3489044003245673No Hit
GGCCGTGATCGTATAGTGGTTAGTACT1289410.3359074313615324No Hit
AGAGAAGTCGGGCTGAAGCTGAGTCCT1251720.3260887149811599No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGG1147260.2988755785233802No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGC1125870.29330321600345005No Hit
AACTCGGATAACCATTGTTAGTTAATC1098730.28623290656956013No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGC1014980.26441498412710324No Hit
GGTTCCATAGTGTAGCGGTTATCACGTCTG1004820.2617681770582631No Hit
GCATGTGGTTCAATGGTAGAATTCTCGCC1002050.2610465574144947No Hit
GCATTGGTGGTTCAATGGTAGAATTCTCGCC1001210.2608277269088031No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAG956500.24918021273086585No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGC951360.24784117844603923No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCCC948020.2469710666734087No Hit
GTTTCTGTAGTGTAGTGGTTATCACGCTC846260.2204613139839232No Hit
TCCCATATGGTCTAGCGGTTAGGATT835690.21770769678730503No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCG756720.19713502412723555No Hit
AGAGTCGCGCAGCGGAAGCGTGCTGGGC737060.19201334824402716No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAA719600.18746479987572512No Hit
GAGTTGCGCAGCGGAAGCGTGCTGGGC715380.18636543709713208No Hit
CGTGATCGTATAGTGGTTAGTACTCTGC714760.18620391934293118No Hit
GTTTCTGTAGTGTAGTGGTTATCACGCT705570.18380980940566338No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCT675400.1759501470762434No Hit
GCATGTGGTTCAGTGGTAGAATTCTCGCC671380.17490288679900545No Hit
AGCAGTGGCGCAGCGGAAGCGTGCTGGGC667330.17384781114656425No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCG666790.17370713439290542No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTC656650.1710655375742008No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCG642900.16748349060603623No Hit
GTTTCCGTAGTGTAGTGGTTATCACGCTC633160.16494609879004185No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCG603180.15713593383690921No Hit
AGGACACAGTGGAGGACTACAAGAGC602230.15688844695547238No Hit
AGAGCGGCAAGAGTGAGATTTCTGAGCT583720.15206636045505595No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTC581790.15156357131697903No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCG557370.14520185590151877No Hit
AGAGTGCGCAGCGGAAGCGTGCTGGGC552270.14387324211696317No Hit
AAAACTATTTGCCAGAGAACTACTAGCC544620.1418803214401298No Hit
AGAGTAGCGCAGCGGAAGCGTGCTGGGC541490.14106491729392218No Hit
GCATTGGTGGTTCAGTGGTAGAATTC538470.14027816952345984No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCG531120.13836340259865915No Hit
GCATTGTTGTTCAATGGTAGAATTCTCGC506990.13207723581016378No Hit
GTTTCCGTAGTGTAGTGGTCATCACGCTCGCC504920.1315379749211383No Hit
GTTTCCGTAGTGTAGTGGTCATCACGCT497130.12950858249335634No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAG477270.12433480410879284No Hit
GCATTGGTGGTTCAGTGGTAGAATTCT463880.12084654164306748No Hit
AAGCAGAACCTGGATCCGTTGTTTGAAC447680.11662623903330269No Hit
AGCAGAACAAGGTCCTGGACACCAAGT440940.11487038473763513No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGC438970.11435717509928718No Hit
CGGACGAGCCGCCCCGGCGGTGAACGGGGAGGAGGCGGGAACCGAAGAAG437630.11400808834020786No Hit
AATCCACCTATAACTTCTCTGTTAACCC435580.11347403770131786No Hit
AGCTAGAAACCCCGAAACCAAACGAGC431400.11238509542299585No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGA426930.1112206045177089No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGG420850.10963668847651321No Hit
TGAATGAGATGAGGGACCAGTATGAGC416550.10851648469737812No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTC412180.10737804504276872No Hit
AGCAGAGTGGCGCAGCGGCAGCGTGCTGGGC406080.10578891875143753No Hit
TGAAAGAGCTAGGAGTAGGACTGGCTC385640.10046404311294418No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCG383930.10001856672635791No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCGCG48500.0354.9159239
CTCCCGC49300.0331.7346538
CGAAGAA117200.0318.1789243
CCGAAGA116000.0316.7102742
GGGACTC92400.0315.9478528
ACCGAAG116800.0305.2128641
TCGCCTC15250.0303.234628
CCTCCCG53900.0291.8541637
GCGAGGA190100.0287.084440
GCGGGAA120550.0281.6666335
AACCGAA124250.0279.03240
TGGGACT287650.0276.7513427
AGCGAGG189400.0275.2017539
GGAGCGA174100.0273.2271737
CGGGAAC129100.0272.8253536
GGGAACC127850.0272.8006337
AGGAGCG180550.0263.4503836
TTCGCCT59450.0249.2452427
CGCGGCG152750.0247.1957941
GAACCGA133250.0246.1954239
Produced by FastQC (version 0.11.5)