FastQCFastQC Report
Tue 28 Feb 2017
fastq1_norrna_nosnrna_notrna.fq

Summary

[OK]Basic Statistics

MeasureValue
Filenamefastq1_norrna_nosnrna_notrna.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27956569
Sequences flagged as poor quality0
Sequence length18-47
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA1950.0547.621341
TTTCGCG11450.0512.015436
TATCTCG15550.0480.7093241
ATTCGCG15850.0464.6036436
TTCGCGT5200.0403.00737
TATTCGC12250.0401.671435
TTCGCGG29250.0333.5230437
TTCTCGT18200.0329.5525537
TCTCGTA13850.0308.4076241
TCGCGTG11900.0302.6813438
TCTCGTG27650.0287.67538
GGGGTTT39050.0281.281137
GGGTTTT44000.0277.988238
ATTTCGC14350.0273.3182735
TTTCTCG24100.0272.9263336
TCGCGAG10250.0264.46141
CGCCAAT17850.0251.6866835
AGTCACG14250.0241.4088330
AATTTCG20700.0236.6997734
CGTGCGA8450.0233.306141