FastQCFastQC Report
Wed 23 Nov 2016
fastq1_norrna_nosnrna_notrna.fq

Summary

[OK]Basic Statistics

MeasureValue
Filenamefastq1_norrna_nosnrna_notrna.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47893052
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG872309918.21370456825345TruSeq Adapter, Index 6 (100% over 49bp)
AAGCTTGGGCTGCAGGTCGACCCGTGCAGATCGGAAGAGCACACGTCTGA8910481.8604953386558032Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGAGATCGGAAGAGCACACGTC3509190.7327137974000906No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACA2900340.605586797851179No Hit
AAGCTTGGGCTGCAGGTCGACCCGTGCGGAGGAGCAGATCGGAAGAGCAC2007120.41908375352650323No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACGT1847770.38581170396073317No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACG1784630.37262816326677195No Hit
AAGCTTGGGCTGCAGGTCGACCCGAGATCGGAAGAGCACACGTCTGAACT1600560.33419461344831397Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGAGCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG1494330.3120139430663137TruSeq Adapter, Index 6 (97% over 49bp)
TGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG1363110.2846153968220693TruSeq Adapter, Index 6 (100% over 49bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGCAGATCGGAAGAGCACAC1363000.28459242898113907No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACAC1286750.2686715392454003No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC1275040.2662265081790987TruSeq Adapter, Index 6 (100% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC1197640.25006550010636197TruSeq Adapter, Index 6 (100% over 50bp)
ACTCTGGACTCTGAATCCAGCGATCCGAGTAGATCGGAAGAGCACACGTC1113890.23257862121628833No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCG1075580.2245795486159454TruSeq Adapter, Index 6 (100% over 50bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC1039660.2170795045594505Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC938060.19586557148205966No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGATCGGAAGAGCACACGTCTG866150.1808508674702961Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT847110.17687534300382446Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAGAGCACACGTCT785990.16411357538876412Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAATACAAGCTTGGGCTGCAGGTCGACCAGATCGGAAGAGCACACGTCTG723400.15104487389945415Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAATACAAGCTTGGGCTGCAGGTCGACAGATCGGAAGAGCACACGTCTGA691180.14431738449243117Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT674070.14074484123500838No Hit
ATATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG661260.13807013175940427TruSeq Adapter, Index 6 (97% over 49bp)
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC627260.13097098092641912Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAGCTTGGGCTGCAGGTCGACCCAGATCGGAAGAGCACACGTCTGAACTC601100.12550881075609882Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCAGATCGGAAGAGCACACGTCT551880.1152317459325833Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGAAGAGCAC547940.11440907963017266No Hit
ACCATCACCTCCAGGGAGATCCAGACGGCAGATCGGAAGAGCACACGTCT541680.11310200068268775Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACAC524980.10961506483236859No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGAAGAGCACACG513360.10718882563591897No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT490010.10231337940208947No Hit
CTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAGAGCACACGTCTG487250.1017370953932942Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG10193100.040.99723444
CTCGTAT10253600.040.75726743
TCTCGTA10251250.040.72366742
GAATACA983900.040.7013741
TATCTCG10220600.040.67280640
ATCTCGT10278750.040.61586841
ATATCTC10250950.040.53843339
AATATCT10262600.040.49091738
CAATATC10294200.040.4086937
CCAATAT10320050.040.349936
GCCAATA10362500.040.2399435
CGCCAAT10366850.040.16976534
TCACGCC10474150.039.99841331
AGTCACG10480150.039.97194729
GTCACGC10481550.039.9699730
CAGTCAC10491700.039.92844828
CACGCCA10492700.039.92325232
CCAGTCA10506650.039.90680727
ACGCCAA10503500.039.8533433
GGGGTAT163800.039.782936