FastQCFastQC Report
Mon 3 Apr 2017
fastq1_norrna_nosnrna_notrna.fq

Summary

[OK]Basic Statistics

MeasureValue
Filenamefastq1_norrna_nosnrna_notrna.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1292539686
Sequences flagged as poor quality0
Sequence length33-40
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGTGTACTCCGAAGAGGATCCAAACTCGTATGCCGTCT69658850.5389300673279289No Hit
GGCATTAACGCGAACTCGGCCTACAATAGTGACTCGTATG51208720.39618682934583416No Hit
AGCGTGTACTCCGAAGAGGATCCAAATCGTATGCCGTCTT46013550.35599332460264593No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATTTCGTATGCC28688390.2219536491663282No Hit
GGCATTAACGCGAACTCGGCCTACAATAGTGATCGTATGC27613680.21363893348184593No Hit
TCGTGGATCCATGTACCCATACGATGTTCCAGATTACGTA14967510.11579922970349708No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGTA2094000.035.12772834
ATTACGT2172850.033.8951633
GCGTGTA19209500.031.4943562
CGTGTAC19554900.031.1381513
GGCATTA19208600.030.8099941
GTGTACT19951900.030.7732894
GTACTCC20242350.030.6697986
AGCGTGT19599650.030.664821
GATTACG2460050.030.60029832
TACTCCG20458150.030.3235847
TGTACTC20240550.030.3110035
GCATTAA19651300.029.8765372
GCCGTCT92317850.029.03007534
CATTAAC20412450.028.704833
TCCGAAG21378250.028.64151410
CGAAGAG21151750.028.63551512
CCATACG4339500.028.62222317
TCCAAAC13551550.028.51734521
ACTCCGA22285650.028.183518
ATCCAAA21645350.028.033420