FastQCFastQC Report
Wed 23 Nov 2016
fastq1_norrna_nosnrna_notrna.fq

Summary

[OK]Basic Statistics

MeasureValue
Filenamefastq1_norrna_nosnrna_notrna.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86903304
Sequences flagged as poor quality0
Sequence length50
%GC31

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGATAACTGTGGTAATTCTAGAGCTAAAAAAAAAAAAAAAAAATCGTAT1726220.1986368665568803No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAAAAAAAAAAAAAAAAAATCGTA1161590.13366465330247973No Hit
AAAATCAGTTATGGTTCCTTTGGTCGAAAAAAAAAAAAAAAAAATCGTAT1130470.13008366172130809No Hit
AAAATCAGTTATGGTTCCTTTGGTCAAAAAAAAAAAAAAAAAATCGTATG938560.10800049673600441No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGG444050.035.5884171
TAGGGGT434550.035.486774
CTAGGGG464700.034.3686033
GGGGTAT460050.033.419466
AGGGGTA463550.033.1433075
GTATGAT467650.033.1175469
TTTTGGT899850.032.986261
GGGTATG470200.032.712977
GGTATGA471550.032.6892978
TCTAGGG491350.032.527722
GTGAGTA417200.032.5083739
CGCAGCG662600.031.9132672
GCGTGCT647700.031.6639912
GGTGAGT430000.031.591468
CGGTTAG122850.031.5344587
AAGCGTG655250.031.45239810
AGCGTGC659450.031.36920411
CCGGAGT310100.030.86856813
AGTATCC471400.030.86775412
TATGATT511900.030.86126510
Produced by FastQC (version 0.11.2)