FastQCFastQC Report
Tue 28 Feb 2017
fastq1_norrna_nosnrna_notrna.fq

Summary

[OK]Basic Statistics

MeasureValue
Filenamefastq1_norrna_nosnrna_notrna.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences207932964
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA15145450.728381383530896Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATCCCACCGCTGCCACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC9324400.4484329863157243No Hit
ATCCCACTCCTGACACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC6215720.29892903368606816No Hit
ATCCCATCCTCGTCGCCACTGTAGGCACCATCAATAGATCGGAAGAGCAC5694470.27386085834855894No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGATC5551590.2669894129917756No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA4816020.23161406961909126No Hit
ACCCCACTCCTGGTACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC4669320.22455891120755628No Hit
ACAAGCTTGGGCTGCAGGTCGACCCGTGCCTGTAGGCACCATCAATAGAT4351640.20928091036109117No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3213190.15453009172706256No Hit
CGAGGGCACGTCTGCCTGGGTGTCACACTGTAGGCACCATCAATAGATCG2471360.11885368978821463No Hit
ACCTCCCGGGAAGTCCTCGTGTTGCATCCCTCTGTAGGCACCATCAATAG2249980.10820698924870806No Hit
ACGCACCTCTGGTGTACCAGTTATCGTGCTGTAGGCACCATCAATAGATC2247850.10810455238833608No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCTT1030100.063.1541181
ATGTACA1674200.062.6415821
ATCCCAC2517950.061.9664761
TGTACAC1676800.061.9139442
GTACACG1677800.061.6552283
TACACGG1741300.059.929944
TGAATAC1614000.058.7928471
CCCACCG1407600.058.430333
CCCACTC1630100.058.3838353
TCCCACC1762100.056.9603922
TCGGGTG513050.056.8722461
ATCCTCG914150.054.7414326
CACTCCT1749800.054.5245255
ACACGGA1912950.054.4683535
CCACTCC1789500.053.7176784
CGCCACT1530100.053.51107814
CGTCGCC1248750.053.00163311
ATCCCAT940250.052.8628771
CACCGCT1573500.052.5750925
GAATACA2046500.052.405172