FastQCFastQC Report
Tue 6 Dec 2016
fastq1_norrna_nosnrna_notrna.fq

Summary

[OK]Basic Statistics

MeasureValue
Filenamefastq1_norrna_nosnrna_notrna.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124300588
Sequences flagged as poor quality0
Sequence length21-75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGG598700.064.247673
CGTTAGC783650.064.051453
TTAGTTC1077900.062.9942865
TTAGCGT1067050.062.7938085
CCGGTTT980250.062.777685
GTTAGTT641350.062.736344
GTTAGGT525700.062.650774
TTAGTTT1256750.062.645215
CTTAGTT1082350.062.5780374
CCGTTAG978300.062.553552
TAGGCGT524650.062.5031363
TTAGGTT948150.062.419395
GGGCGTT433750.062.144044
CGTTTAG922900.062.1420632
TGTTAGC809600.062.1400153
GTTTAGT760750.062.139153
TTAGCTT1177450.062.1327825
AATGCGT523800.062.0775645
GTTAGCT622300.062.0042534
CGTTAGT815300.061.9908073