FastQCFastQC Report
Mon 3 Apr 2017
fastq1_norrna_nosnrna_notrna.fq

Summary

[OK]Basic Statistics

MeasureValue
Filenamefastq1_norrna_nosnrna_notrna.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25311926
Sequences flagged as poor quality0
Sequence length36
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGTGTACTCCGAAGAGGATCCAAATCGTATGCCG2059640.813703390251694No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN817310.3228952233820532No Hit
AGCGTGTACTCCGAAGAGGATCCAAACTCGTATGCC742200.2932214640640147No Hit
GGCATTAACGCGAACTCGGCCTACAATAGTGACTCG423040.16713070352686712No Hit
GGCATTAACGCGAACTCGGCCTACAATAGTGATCGT270960.10704835341253764No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGTA399500.028.4361272
CGTGTAC409250.027.9540023
GTACTCC409500.027.7504886
TACTCCG406050.027.7312017
ACTCCGA412250.027.3640658
AGCGTGT414800.027.3244671
GGCATTA172550.027.0058611
CTCCGAA415050.026.9677019
TGTACTC421650.026.9455475
TCCGAAG412050.026.71761110
GCATTAA175750.026.6249222
GTGTACT428350.026.5625134
CGAAGAG410150.026.53574412
CCGAAGA419600.026.2838211
TCCAAAT320950.025.81050321
ATCCAAA433350.025.7230620
GATCCAA437250.025.62976519
ATTAACG177300.025.4141944
CATTAAC185500.025.0439363
AGGATCC440800.025.03094317