fastq1_norrna_nosnrna_notrna.fq FastQC Report

Basic Statistics

Measure	Value
Filename	fastq1_norrna_nosnrna_notrna.fq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20563572
Sequences flagged as poor quality	0
Sequence length	50
%GC	50

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATGATCG	594440	2.8907429117859484	No Hit
CTGTAGGCACCATCAATGATCGGAAGAGCACACGTCTGAACTCCAGTCAC	140378	0.6826537724088013	Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA	135751	0.6601528178081123	No Hit
TGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATGATCGG	53269	0.2590454615569707	No Hit
ACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATGATCGGAAG	36033	0.17522733890785122	No Hit
ATGTCCACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATGATCG	28529	0.13873562433608325	No Hit
TACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATGATCGGAA	24654	0.11989162194194668	No Hit
ACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAATGATCG	21140	0.10280315112568963	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
ATGTACA	102635	42.04614	1
TCTCCCG	14185	39.977497	1
TCGTTTT	14065	39.723816	1
TGTACAC	120565	38.981174	2
GTACACG	121785	38.37761	3
TACACGG	127755	36.44824	4
CTGTACA	11750	34.960117	1
ACACGGA	134650	34.387493	5
CAGTCAC	26315	33.90207	44
CACGGAG	144530	33.789555	6
ACGGAGT	148130	32.93928	7
AGTCGAC	116190	32.793114	11
GTCGACC	116205	32.644962	12
CCGCCGA	9955	32.617435	1
CGACCCG	114890	32.50338	14
CCAGTCA	27465	32.49024	43
TCGACCC	116630	32.30148	13
GACCCGC	116220	32.159534	15
CGGAGTC	151205	32.053818	8
GGGCGCA	12125	31.978207	8