FastQCFastQC Report
Tue 28 Feb 2017
fastq1_norrna_nosnrna_notrna.fq

Summary

[OK]Basic Statistics

MeasureValue
Filenamefastq1_norrna_nosnrna_notrna.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60288929
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG11708461.9420580518190993No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA3521240.5840607982934977No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2604530.43200800598066685Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCGTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA1607580.26664597077184765Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCCTGCCGACTACGCCACTGTAGGCACCATCAATAGATCGGAAGAGCAC1537760.25506507173149484No Hit
TCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1415420.23477278888135497Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACATATACTAAAATTGGAACGATACAGAGAACTGTAGGCACCATCAATAG1373410.22780467704111976No Hit
AATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC1229130.20387325175406584Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCCCACCGCTGCCACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC912330.15132629076890716No Hit
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC876460.14537660803362423Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA842480.13974041569058226Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACGATATCT752530.12482059517096415TruSeq Adapter, Index 7 (97% over 35bp)
CCTCGACACACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA742480.12315362245031755Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC742270.12311879018451297Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG731190.12128097349349165Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGTGGCGTACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG698080.11578908625164Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA686730.11390648521886995Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCCCATCCTCGTCGCCACTGTAGGCACCATCAATAGATCGGAAGAGCAC683490.11336907311788537No Hit
CCGTCTCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC615060.10201873050357223Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT613490.10175831784970006Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTACA2200300.041.5612831
GTACACG2212750.041.005513
TGTACAC2231550.040.8184432
TACACGG2241700.040.3685264
CCGACTA236200.038.9671367
ACACGGA2348900.038.424285
CACGGAG2369150.037.9654736
GACTACG250450.036.9698269
ACTACGC249600.036.8581610
ACGGAGT2441150.036.699287
TACGCCA249450.036.54594812
CGGAGTC2455000.036.346018
GAGTCGA2477950.036.063110
GGAGTCG2484600.035.9011659
AGTCGAC1892100.035.64671711
GTCGACC1896950.035.50003412
CGCCACT409500.035.42652514
CGACCCA1903850.035.27654314
ACGCCAC261450.035.23901713
CGACTAC262000.035.1505178