FastQCFastQC Report
Tue 13 Dec 2016
fastq1_norrna_nosnrna_notrna.fq

Summary

[OK]Basic Statistics

MeasureValue
Filenamefastq1_norrna_nosnrna_notrna.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22008187
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA820576137.28503851771162Illumina PCR Primer Index 6 (96% over 29bp)
CTGTCGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA5475392.487887802843551Illumina PCR Primer Index 6 (100% over 28bp)
TAGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATATCTC4749272.157956036996596No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGACTGCTGTAGGCACCATCAAT1776640.8072632243628246No Hit
TTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1210040.5498135761932593Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAT972840.4420355025154957Illumina PCR Primer Index 6 (96% over 29bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA804680.365627573048157Illumina PCR Primer Index 6 (100% over 28bp)
CGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA758340.34457177231363945Illumina PCR Primer Index 6 (96% over 29bp)
CAGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATATCTC704100.3199263982989603No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAC514340.23370393935674938Illumina PCR Primer Index 6 (96% over 29bp)
GAGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATATCTC453400.20601424369940147No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAATA445950.20262913978329974Illumina PCR Primer Index 6 (96% over 29bp)
CCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA395840.1798603401543253Illumina PCR Primer Index 6 (100% over 28bp)
AGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATATCTCG356590.16202606784466161No Hit
CTGTAGGCACTATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA267410.12150478365164745Illumina PCR Primer Index 6 (100% over 28bp)
CTGTAGGCATCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA266500.12109130115988201Illumina PCR Primer Index 6 (96% over 29bp)
GACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA265340.12056422457697219Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGTACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA261920.11901025741011743Illumina PCR Primer Index 6 (100% over 28bp)
ATGTACACGGCGTCGAGCACCCGCAACGCGACTGCTGTAGGCACCATCAAT236220.10733278484047776No Hit
ATGTACACGGAGTCGAGCACCCGCACCGCGACTGCTGTAGGCACCATCAAT233070.10590149929205891No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGCA620050.044.1837653
TAGTCAC565750.044.1341321
CTGTCGG618000.043.8488541
TCGGCAC623350.043.827144
TGTCGGC625700.043.798762
CTCGTAT10184000.042.15650620
ATGCCGT10225500.042.10691525
CGTATGC10232350.042.10573622
TCTCGTA10192900.042.10338219
TCGTATG10232500.042.10006321
TATGCCG10232800.042.09604624
GTATGCC10241800.042.0666723
CTTGAAA10213950.042.0658738
GCTTGAA10216000.042.0650937
TGCTTGA10220200.042.0459836
CCGTCTT10234950.042.04191228
TTGAAAA10221200.042.0336439
TGAAAAA10222350.042.02636740
GAAAAAA10219200.042.0244541
CGTCTTC10238000.042.01663629