FastQCFastQC Report
Tue 11 Apr 2017
fastq1_norrna_nosnrna_notrna.fq

Summary

[OK]Basic Statistics

MeasureValue
Filenamefastq1_norrna_nosnrna_notrna.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33509668
Sequences flagged as poor quality0
Sequence length40
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGTA2006900.031.29301810
CGTGTAC2015850.031.2148311
GTGTACT2026650.031.10386712
TGTACTC2029650.031.06729913
GTACTCC2036050.031.03312514
TACTCCG2041350.030.93339215
CTCCGAA2047700.030.72965217
ACTCCGA2056650.030.7032716
AGCGTGT2045950.030.6731789
GGCATTA2601700.030.5717959
TCCGAAG2060100.030.4245718
GGATCCA2079150.030.16966626
CCGAAGA2080400.030.09476919
GATCCAA2091150.030.07628327
TCCAAAT1687350.030.07295629
ATCCAAA2100100.029.96993428
AGGATCC2107050.029.8165425
TAGGCAT407300.029.7037057
ATCGTAT1804050.029.70150634
AGAGGAT2108650.029.68380223