FastQCFastQC Report
Mon 3 Apr 2017
fastq1_norrna_nosnrna_notrna.fq

Summary

[OK]Basic Statistics

MeasureValue
Filenamefastq1_norrna_nosnrna_notrna.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74590290
Sequences flagged as poor quality0
Sequence length25-44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGAGTCGACCCGCAACGCGA57299917.6819529726992615No Hit
ATGTACACGGAGTCGACCCGCAACGCG54167077.26194656167713No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCG4724350.6333733251338747No Hit
TTAACGCGAACTCGGCCTACAATAGTGA3120900.41840566647481864No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGG3026920.4058061712858336No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC2910690.39022371410541506No Hit
TTAACGCGAACTCGGCCTACAATAGT2860460.3834895936186868No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGG2812960.3771214725133794No Hit
TCGCCGCGGCCCGGGTTCGTTTCCCGGTCAGG2390780.32052161212940716No Hit
ATGTACACGGAGTCGACCCGCAACGC2279480.30560009888686585No Hit
TTGGTCTAGGGGTATGATTCTCGCTT2170870.29103922239744606No Hit
GCATTGGTGGTTCAGTGGTAGAATTCT1994510.2673953942262458No Hit
TTTCCGTAGTGTAGTGGTTATCACGTTCG1832060.24561642004609446No Hit
TTTGGTGGTTCAGTGGTAGAATTCTCGC1817500.24366442334518343No Hit
TCGGGAGACCGGGGTTCGATTCCCCGACGGG1751040.23475441642605224No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCG1725880.2313813232258515No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG1678840.22507487234598497No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCG1491570.19996838730617617No Hit
ATTAACGCGAACTCGGCCTACAATAGTGA1432130.19199952165355572No Hit
ATGGACACGGAGTCGACCCGCAACGCG1363900.1828522184321847No Hit
TACACGGAGTCGACCCGCAACGCGA1360100.1823427687437601No Hit
ATGGACACGGAGTCGACCCGCAACGCGA1348060.180728617625699No Hit
AGGTACACGGAGTCGACCCGCAACGCG1301330.17446372711515132No Hit
AGGTACACGGAGTCGACCCGCAACGCGA1292550.17328663020347554No Hit
ACGCGAACTCGGCCTACAATAGTGA1273750.1707661949028486No Hit
TTCCCTGGTGGTCTAGTGGTTAGGATTCG1273570.1707420630755022No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAG1200740.16097805759972242No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG1197370.1605262561655143No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG1185930.15899254447194133No Hit
TTTCCGTAGTGTAGTGGTTATCACGTTCGC1184670.15882362168051634No Hit
TCCGTAGTGTAGTGGTTATCACGTTCGC1152200.15447050815863567No Hit
TATGTACACGGAGTCGACCCGCAACGCG1124020.15069253652184486No Hit
TATGTACACGGAGTCGACCCGCAACGCGA1103110.14788922257843481No Hit
ATTAACGCGAACTCGGCCTACAATAGT1087630.14581388542664198No Hit
TTTGGTCTAGGGGTATGATTCTCGCTT977230.13101303132083278No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGG899420.12058137862180185No Hit
TGTACACGGAGTCGACCCGCAACGCGA876440.11750054866390787No Hit
TCGGGAGACCGGGGTTCGATTCCCCGACGGGG829310.11118203187036813No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTC827210.11090049388465979No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGG812160.10888280498708344No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATG810660.1086817064258632No Hit
TGTACACGGAGTCGACCCGCAACGCG777840.10428166990636449No Hit
TTCCCTGGTGGTCTAGTGGTTAGGATTCGG765290.10259914527748852No Hit
TTCCGTAGTGTAGTGGTTATCACGTTCGC757930.10161242167043459No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAGAT64150.06428.832537
CCGTCCG28700.03592.41837
AATAGTA35250.02924.890637
GCCCGTC35609.1904175E-42896.134837
TATGCAA37200.00100349952771.569837
GCGTACG5650.0010963022721.224935
ACGCCCT37900.00104161682720.380137
TGGTTTA38750.00108885712660.70737
ACGGCGC40350.00118062112555.20237
TATCAGA54151.8189894E-122312.017836
CGCCCGT16250.00216803891926.088636
AGGTCTC66100.00316783531559.794237
TACGCCC21750.00388355791439.031936
CGTACGG22150.01413.044836
TTGGGGC81500.01265.0637
TTATCAG54206.730261E-111134.680435
ACTTCTT94250.0064395071093.924737
CGGACTG33052.3038592E-7930.403635
TTACGCC18000.0854.162235
CCCGTCC45651.6225385E-8685.628636