FastQCFastQC Report
Mon 3 Apr 2017
fastq1_norrna_nosnrna_notrna.fq

Summary

[OK]Basic Statistics

MeasureValue
Filenamefastq1_norrna_nosnrna_notrna.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4826302
Sequences flagged as poor quality0
Sequence length26-48
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGAGTCGACCCGCAACGC4508529.341562131835099No Hit
ATGTACACGGAGTCGACCCGCAACGCG2601535.390317472880893No Hit
ATGTACACGGAGTCGACCCGCAACGCC181080.3751940927028603No Hit
ATGTACACGGAGTCGACCCGCAACGTG118910.24637911179200972No Hit
ATGTACACGGAGTCGACCCGCAACGCT95070.19698311460824455No Hit
ATGTACACGGAGTCGACCCGCAACGCGG94850.19652727906376352No Hit
ATGTACACGGAGTCGACCCGCAACGCAAA62690.12989241037962398No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTTGA2901.2400864E-518174.14642
ACCCCCC1355.6431836E-511154.50338
CCCCCCA2458.8505025E-58604.90139
CCCAACC6205.668123E-58500.81142
GCCCCCC2851.19762844E-47397.19739
CCCCCAA5301.2425514E-46629.56340
CCCCAAC9901.4451945E-45323.739741
CTGCTTG10100.05218.319341
CGCCCCC2952.6945033E-45104.60338
AAAAACC4801.0069925E-84392.085439
ACGCCCC2504.146613E-44216.40237
CCCACGC2605.48152E-43685.66636
TAAAAAC2806.3571933E-43422.404336
AGCCCCC4456.131039E-43383.950438
CACGCCC2856.586244E-43362.362336
ACAAAAC4957.586084E-43042.13738
ATTAGAC1108.847739E-42994.603833
TCTGCTT12750.02755.818440
CACCCCC4450.00131368322368.765137
CGTAGCG700.00145620762352.90332