FastQCFastQC Report
Mon 15 May 2017
fastq1_norrna_nosnrna_notrna.fq

Summary

[OK]Basic Statistics

MeasureValue
Filenamefastq1_norrna_nosnrna_notrna.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67257554
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTC3867140.5749748199287771No Hit
AAGGGTCAGGGGGCGAAGGTGGCCCGTCGGAGATCGGAAGAGCACACGTC3279230.4875630773013244No Hit
TCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTCTGA1580090.23493123166507066Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGGTCAGGGGGCGAAGGTGGCCCGTCGGAGATCGGAAGAGCACACGTCT1005240.14946127835692627Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCCGCGGACGGTGTGAGGCCGGTGTCGAGATCGGAAGAGCACACGTCT929460.1381941424750594Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGAACCATCTAGTAGCTGGTTCCCTCCGAAGAGATCGGAAGAGCACACGT771740.11474398845964574No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGACC178800.064.8581161
CGGATCT644900.064.1738741
AGCCCGC661750.061.6183931
TCCGCGC1287750.058.4911725
CCGCGCA649750.057.7793426
CGCGCCA448650.057.72962627
TGCCCGC83800.056.3477021
CTCCGCG1775300.055.85224
CCTCCGC1834250.055.79420523
ACGCGAC33050.055.6277774
CTGCTTG1483650.055.4008558
TGCTTGA1487800.055.30267759
GCTTGAA1490000.055.1999260
CTTCTGC1492950.055.0510655
TCTGCTT1496350.054.97033757
AACGATT77800.054.958481
TTCTGCT1497550.054.90295856
GTCTTCT1496700.054.80975753
AGCGCCG4989950.054.761851
CCCCCTG206850.054.3913571