FastQCFastQC Report
Thu 17 Aug 2017
fastq1_norrna_nosnrna_notrna.fq

Summary

[OK]Basic Statistics

MeasureValue
Filenamefastq1_norrna_nosnrna_notrna.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143993331
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTCTAGCATAACAAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG24688921.7145877401780503No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA13352600.927306834786675Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCTCTAGCATAACAAGTATTAGAGGCCTGTAGGCACCATCAATAGATCGG11430420.7938159302669372No Hit
GCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATAGATCGGAAGA10969760.7618241708708023No Hit
ACGATTAAAGTCCTACGTGATCTGAGTCTGTAGGCACCATCAATAGATCG8858910.6152305761993936No Hit
GCGCAGCGGAAGCGTGCTGGGCTGTAGGCACCATCAATAGATCGGAAGAG5885690.4087474023362929No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA5499970.38196005063595617No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG5479860.38056345817849024No Hit
ACATATACTAAAATTGGAACGATACAGAGAACTGTAGGCACCATCAATAG4446670.30881082957932265No Hit
TCCTCTAGCATAACAAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG3481550.24178550324667467No Hit
ACGATTAAAGTCCTACGTGATCTGAGTTCTGTAGGCACCATCAATAGATC3306920.22965785825178253No Hit
CTGTAGGCACCATCAATAGATTGGAAGAGCACACGTCTGAACTCCAGTCA3266580.22685633961756188Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
ACCTCTAGCATAACAAGTATTAGAGGCCTGTAGGCACCATCAATAGATTG2668860.18534608384050785No Hit
TACCTCTAGCATAACAAGTATTAGAGGCCTGTAGGCACCATCAATAGATC2407230.1671764923613025No Hit
ATCCTGCCGACTACGCCACTGTAGGCACCATCAATAGATCGGAAGAGCAC2403670.1669292586890708No Hit
GCGCAGCGGAAGCGTGCTGGGCCCTGTAGGCACCATCAATAGATCGGAAG2324590.16143733767781232No Hit
TATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCG1937810.134576371457092No Hit
TGAGGGCATCAAGCACATAATATAGCTCAAGACGCCCTGTAGGCACCATC1899720.13193111005953465No Hit
ACGATTAAAGTCCTACGTGATCTGAGCTGTAGGCACCATCAATAGATCGG1800170.12501759543294405No Hit
ACGATTAAAGTCCTACGTGATCTGAGTTCAGACCGCTGTAGGCACCATCA1776120.12334737919216551No Hit
GCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATAGATTGGAAGA1768710.12283277202608778No Hit
TCGATTAAAGTCCTACGTGATCTGAGTCTGTAGGCACCATCAATAGATCG1590920.11048567242325966No Hit
AGGTAAGCATACTGGAAAGTGTGCTTGCTGTAGGCACCATCAATAGATCG1533160.10647437553896161No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATTA544150.041.826381
CGCAGCG3906150.039.4464842
GCGCAGC3823400.039.073671
CAGCGGA3902200.038.7530824
GCAGCGG3966800.038.6982573
GGAAGCG3894050.038.6832588
AGCGGAA3929950.038.622185
ACACGTC2517950.038.54528431
CGTCTGA2483300.038.5259134
GCGGAAG3916850.038.476996
GAAGCGT3921250.038.440769
AAGCGTG3880100.038.42265710
ACATATA1061150.038.1890831
CACACGT2541350.038.1626730
AGCGTGC3954850.038.10916511
CGGCTAG426100.038.101564
CCAGTCA2502750.038.0120344
CGGAAGC3987950.037.8639567
GCGTGCT4030750.037.32716412
CACGTCT2609550.037.110532