FastQCFastQC Report
Tue 28 Feb 2017
fastq1_norrna_nosnrna_notrna.fq

Summary

[OK]Basic Statistics

MeasureValue
Filenamefastq1_norrna_nosnrna_notrna.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences824550722
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGGCATACGAGATATTCCGGTGACTGGAATTCTCGGGTGCCAAGGAACTC653331367.923482965551269RNA PCR Primer, Index 37 (96% over 33bp)
CATACGAGATATTCCGGTGACTGGAATTCTCGGGTGCCAAGGAACTCCAGT427125595.180100854972024RNA PCR Primer, Index 1 (100% over 30bp)
CGGCATACGAGATATTCCGGTGACTGGAATTCTCGGGTGCCAAGGAACTCC222021822.6926399319798304RNA PCR Primer, Index 37 (96% over 32bp)
GACGGCATACGAGATATTCCGGTGACTGGAATTCTCGGGTGCCAAGGAACT186298392.2593927217493848RNA PCR Primer, Index 37 (97% over 34bp)
AGACGGCATACGAGATATTCCGGTGACTGGAATTCTCGGGTGCCAAGGAAC124867171.5143661471440686RNA PCR Primer, Index 37 (97% over 35bp)
CGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGGAA77639170.9415936209682926RNA PCR Primer, Index 1 (100% over 23bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGG75077680.9105283398199486Illumina Small RNA Adapter 2 (100% over 21bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGGA65931790.7996086625220371RNA PCR Primer, Index 1 (100% over 22bp)
GGTCGCTGGTTCGTTTCCGGCTCGAAGGTGGAATTCTCGGGTGCCAAGGAA56642380.6869484009741732RNA PCR Primer, Index 1 (100% over 23bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGTTGGAATTCTCGGGTGCCAAG55039220.6675055703850321No Hit
GGCATACGAGATATTCCGGTGACTGGAATTCTCGGGTGCCAAGGAACTCCA39699160.48146413484069456RNA PCR Primer, Index 37 (96% over 31bp)
GAATACAAGCTTGGGCTGCAGGTCGACTGGAATTCTCGGGTGCCAAGGAAC34829660.4224077315161214RNA PCR Primer, Index 1 (100% over 24bp)
ACGGCATACGAGATATTCCGGTGACTGGAATTCTCGGGGGCCAAGGAACTC31748870.38504447516571333RNA PCR Primer, Index 37 (96% over 33bp)
AGGTCGCTGGTTCGTTTCCGGCTCGAAGGTGGAATTCTCGGGTGCCAAGGA30829300.37389209878103774RNA PCR Primer, Index 1 (100% over 22bp)
TCTTTGGTCTAGGGGTATGATTCTCGCTTTGGAATTCTCGGGTGCCAAGGA30577840.37084243799861716RNA PCR Primer, Index 1 (100% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCTGGAATTCTCGGGTGCCAAGGAAC29979100.3635810290394725RNA PCR Primer, Index 1 (100% over 24bp)
GAATACAAGCTTGGGCTGCAGGTCGACCTGGAATTCTCGGGTGCCAAGGAA29501570.3577896327401433RNA PCR Primer, Index 1 (100% over 23bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGGAATTCTCGGGTGCCAAGG27760040.33666867615695323Illumina Small RNA Adapter 2 (100% over 21bp)
ATACGAGATATTCCGGTGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC24665290.2991361154856887RNA PCR Primer, Index 1 (100% over 31bp)
GTCGCTGGTTCGTTTCCGGCTCGAAGGTGGAATTCTCGGGTGCCAAGGAAC21175390.2568112480532156RNA PCR Primer, Index 1 (100% over 24bp)
GGTCGCTGGTTCGTATCCGGCTCGAAGGTGGAATTCTCGGGTGCCAAGGAA19432120.2356691890690019RNA PCR Primer, Index 1 (100% over 23bp)
TTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGGAAC18606870.22566070835360932RNA PCR Primer, Index 1 (100% over 24bp)
TCTTGGTCTAGGGGTATGATTCTCGCTTTGGAATTCTCGGGTGCCAAGGAA18434580.22357120681776566RNA PCR Primer, Index 1 (100% over 23bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGA17514490.21241252396841634RNA PCR Primer, Index 1 (100% over 22bp)
CGGCATACGAGATATTCCGGTGACTGGAATTCTCGGGGGCCAAGGAACTCC14957480.1814015754388RNA PCR Primer, Index 37 (96% over 32bp)
GCATACGAGATATTCCGGTGACTGGAATTCTCGGGTGCCAAGGAACTCCAG14784900.17930855683611907RNA PCR Primer, Index 37 (96% over 30bp)
GGTCGCTGGTTCGTATCCGGCTCGGAGGTGGAATTCTCGGGTGCCAAGGAA13115560.1590631073390777RNA PCR Primer, Index 1 (100% over 23bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAGG12814970.15541760692315543Illumina Small RNA Adapter 2 (100% over 21bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGAA12317920.14938947564222738RNA PCR Primer, Index 1 (100% over 23bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCTGGAATTCTCGGGTGCCAAGGA12164170.1475248238276359RNA PCR Primer, Index 1 (100% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGTGGAATTCTCGGGTGCCAAGGAA11614510.1408586481111589RNA PCR Primer, Index 1 (100% over 23bp)
TCCCTGTGGTCTAGTGGTTAGGATTCTGGAATTCTCGGGTGCCAAGGAACT11322840.13732132782002462RNA PCR Primer, Index 1 (100% over 25bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGAA11202940.1358672026000603RNA PCR Primer, Index 1 (100% over 23bp)
CCATACGAGATATTCCGGTGACTGGAATTCTCGGGTGCCAAGGAACTCCAG10696000.12971912721216441RNA PCR Primer, Index 1 (100% over 29bp)
GACGGCATACGAGATATTCCGGTGACTGGAATTCTCGGGGGCCAAGGAACT10655010.12922200800644015RNA PCR Primer, Index 37 (97% over 34bp)
ATGTGTGACTGAAAGGTATTTTCTGAGCTGGAATTCTCGGGTGCCAAGGAA10515730.1275328456992122RNA PCR Primer, Index 1 (100% over 23bp)
CCCATGGTGTAATGGTTAGCACTCTGGTGGAATTCTCGGGTGCCAAGGAAC10078090.12222522800725898RNA PCR Primer, Index 1 (100% over 24bp)
CCTGGAATTCTCGGGTGCCAAGGCTGGAATTCTCGGGTGCCAAGGAACTCC9998660.12126191552834516RNA PCR Primer, Index 1 (100% over 27bp)
CAAGCAGAAGACGGCATACGAGATATTCCGGTGACTGGAATTCTCGGGTGC9896160.1200188143186175RNA PCR Primer, Index 37 (97% over 43bp)
CCGGCATACGAGATATTCCGGTGACTGGAATTCTCGGGTGCCAAGGAACTC9674170.11732656029376444RNA PCR Primer, Index 37 (96% over 29bp)
CACGGCATACGAGATATTCCGGTGACTGGAATTCTCGGGTGCCAAGGAACT9525950.11552897530541487RNA PCR Primer, Index 37 (96% over 30bp)
CGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAGGA8713600.1056769434251978RNA PCR Primer, Index 1 (100% over 22bp)
GGTCGCTGGTTCGAATCCGGCTCGAAGGTGGAATTCTCGGGTGCCAAGGAA8675700.10521729917301557RNA PCR Primer, Index 1 (100% over 23bp)
TCCCATGGTGTAATGGTTAGCACTCTGGTGGAATTCTCGGGTGCCAAGGAA8294810.10059793507766768RNA PCR Primer, Index 1 (100% over 23bp)
CTTGGAATTCTCGGGTGCCAAGGCTGGAATTCTCGGGTGCCAAGGAACTCC8282560.10044936932333436RNA PCR Primer, Index 1 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATACA19840250.043.659591
AATACAA20449700.043.3664782
TACAAGC20557950.043.05154
ACCCGTT6667550.042.8911126
TCCCATA2266050.042.705431
GGTCGAC19972800.042.6291121
ATACAAG20819450.042.569213
AGGTCGA20750000.042.20265220
GTCGACT4425550.042.1637922
GCTTGGG21136350.042.1463669
CAAGCTT21175300.042.0409246
CAGGTCG20881050.041.99806619
CCCGTTG6813450.041.91716427
CTTGGGC21287850.041.83320210
GCCGTGA10469350.041.7610361
GTCGACC15902450.041.55142622
TGCAGGT21287200.041.48287617
ACAAGCT21554150.041.4499555
GCAGGTC21606100.040.74501818
TCGACCC12364150.040.61671423