FastQCFastQC Report
Wed 4 Jan 2017
fastq1_norrna_nosnrna_notrna.fq

Summary

[OK]Basic Statistics

MeasureValue
Filenamefastq1_norrna_nosnrna_notrna.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121243155
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG7877310.6497117301178775No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA4350100.35879138908914077No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGCTGTAGGCACCATCAAT2817930.2324197188699024No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCTGTAGGCACCATCAATAGATC2814410.23212939320162032No Hit
TTTGGTCTAGGGGTATGATTCTCGCTTCTGTAGGCACCATCAATAGATCG2630910.2169945181647574No Hit
ATCCTGCCGACTACGCCACTGTAGGCACCATCAATAGATCGGAAGAGCAC1799430.14841497649908567No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGTAGGCACCATCAATAGAT1473630.12154335640638847No Hit
GTCTAGGGGTATGATTCTCGCTTCTGTAGGCACCATCAATAGATCGGAAG1422750.1173468308375842No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGCTGTAGGCACCATCAATA1400100.1154786841368488No Hit
TTGGTCTAGGGGTATGATTCTCGCTTCTGTAGGCACCATCAATAGATCGG1338020.11035839507805617No Hit
TATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCG1310320.10807373001799567No Hit
GTATAGTGGTGAGTATCCCCGCCTGTAGGCACCATCAATAGATCGGAAGA1295720.1068695383256894No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGC1128550.037.916661
TCTCCCG644800.037.1914251
GTACACG2215600.037.1881983
TGTACAC2273750.036.3486862
TACACGG2259100.036.21584
CCCTGGT1808450.035.9179422
ATGTACA2312900.035.905431
GGTGGTC1777250.035.8457576
TAGCCTC454500.035.8351
CGGCTAG371100.035.6487924
TCCCTGG1741200.035.545321
TATGTAC317850.034.3826031
CCGGCTA403950.033.8672263
ACACGGA2401300.033.7139555
CCGACTA343450.033.337267
ACTACGC342350.033.174710
CGACCCA1635750.032.89410814
TACGCCA347900.032.8227712
CTACGCC1687550.032.78272211
CACGGAG2520300.032.575076