FastQCFastQC Report
Fri 7 Jul 2017
fastq1_norrna_nosnrna_notrna.fq

Summary

[OK]Basic Statistics

MeasureValue
Filenamefastq1_norrna_nosnrna_notrna.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37623500
Sequences flagged as poor quality0
Sequence length36
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTT1391060.36973168365516235No Hit
ACAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTT1310970.34844445625739234No Hit
TCAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTT846150.22489933153481204No Hit
GTAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTT654300.1739072654059298No Hit
GCAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTT609730.1620609459513336No Hit
ATAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTC605120.16083564793280794No Hit
TTAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTT602110.1600356160378487No Hit
ACAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTC577640.15353170226055524No Hit
AGAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTT519160.13798822544420375No Hit
ACAAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCT429940.11427432322883303No Hit
TGAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTT403470.10723882679708162No Hit
TCAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTC381140.10130370646005821No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG3700.027.55885516
GTAGGTA5100.020.2773215
GCGGTCC15850.019.9609742
GGGTAGT9850.019.4731211
CTAGGGT5200.019.03315417
AGGGGGG1950.018.4538144
CGTCTTT232700.018.03996730
CCCCCTT35450.017.8007730
CCCCTTT27050.017.66304630
CCGTTTT258850.017.2042530
AGTAGGT6150.017.05763214
ATAGGTC3550.016.9015751
GGGGGTG2350.015.9426496
TAGGTCC3500.015.8512042
CGGGGCG3900.015.7545869
TAGGGTT7550.015.4930918
TAGGGGT3700.015.40182818
ACCCGTA54900.014.9943822
TAAGTAG2250.014.6611742
GGCTAGG8300.014.62643815