FastQCFastQC Report
Wed 23 Nov 2016
fastq1_norrna_nosnrna_notrna.fq

Summary

[OK]Basic Statistics

MeasureValue
Filenamefastq1_norrna_nosnrna_notrna.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21465395
Sequences flagged as poor quality0
Sequence length40
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACC1907180.8884905216046571No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAA1672450.7791377703508368Illumina Single End Adapter 1 (95% over 22bp)
AGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCA976270.4548111041050025No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGACTGCTGTAG791200.3685932637158552No Hit
TGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAA540390.2517493854643718Illumina Single End Adapter 1 (95% over 22bp)
ACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTG466800.21746629866350004Illumina Single End Adapter 1 (95% over 22bp)
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGA309810.14432997855385377Illumina Single End Adapter 1 (95% over 22bp)
GTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCAT219480.10224829312481787No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCAC272050.031.1219941
GTACACG201900.027.4071353
TGATACG29350.025.990853
TACACGG213750.025.617424
ACACGGA227600.024.028685
ATACGGC31850.024.0040385
GATACGG32300.023.7222144
CACGGAG238450.022.8711996
GCGATAA45850.022.34755320
AGTCACT461450.021.9945492
CGCCTTA50400.021.87916612
ACGGAGT246500.021.8623817
CGGAGTC248100.021.6118268
TAAGCGA51500.021.31634517
TTAAGCG52500.021.2016416
CACTTAG480800.020.936555
TCACTTA481350.020.9232164
GTCACTT487800.020.8973733
CTTAGCG481000.020.6382087
GCTTGAA309450.020.62691534