FastQCFastQC Report
Tue 28 Feb 2017
fastq1_norrna_nosnrna_notrna.fq

Summary

[OK]Basic Statistics

MeasureValue
Filenamefastq1_norrna_nosnrna_notrna.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1004523
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATGC254.4424378E-544.05
ACGCGTC207.856008E-444.018
TAGGACG404.123849E-738.51
CCTTTTC301.3009788E-436.6666646
CCCTTTT301.3009788E-436.6666645
GGTGCGA601.9826984E-1036.66666441
CGTGTAT301.3009788E-436.6666641
CGCCAAT301.3009788E-436.66666424
AACGTTA505.9442755E-835.25
TCCATAG250.00235260435.21
CGACGGT1250.035.223
TACGCGT250.00235260435.217
GTATGGT250.00235260435.218
TAGGGAG250.00235260435.21
CCGACCC505.9442755E-835.22
TCGCGGC250.00235260435.211
GCGACGG2400.034.83333222
GCGCACA702.7284841E-1134.571433
ACGTTAT451.0369822E-634.222226
GACCGGT451.0369822E-634.2222223