FastQCFastQC Report
Fri 13 Jan 2017
fastq1_norrna_nosnrna_notrna.fq

Summary

[OK]Basic Statistics

MeasureValue
Filenamefastq1_norrna_nosnrna_notrna.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72949823
Sequences flagged as poor quality0
Sequence length26-36
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGAGTCGACCCGCAACGCGA16771592.2990583541237655No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGACTG9885701.35513694118216No Hit
GCTGGTCCGATGGTAGTGGGTTATCAGAA3174180.4351182592999574No Hit
ACGGAGTCGAGCACCCGCAACGCGACTG2958590.40556506902011263No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAA2431540.33331677857532294No Hit
TCGCCGCTGGTGTAGTGGTATCATGCAAGATT2172450.29780058547914506No Hit
TGCTGGTCCGATGGTAGTGGGTTATCAGAA1558760.21367563839051398No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGG1474610.202140312252711No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGA1134130.15546713526638714No Hit
TACACGGAGTCGAGCACCCGCAACGCGACTG1109840.15213744932595655No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATT1048330.14370562626313704No Hit
ACACGGAGTCGAGCACCCGCAACGCGACTG984680.13498045087785887No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGACT949070.13009901339993657No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCG775090.1062497437450945No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATT756360.10368222552095843No Hit
TGTACACGGAGTCGAGCACCCGCAACGCGACTG734640.1007048365285273No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACAGTT146450.0317.1300430
CCAGCTA132350.0242.9097930
CGACAGT122550.0213.4390329
CCCAGCT343350.0188.2243730
GTCGCCA117250.0177.9684330
AGGTATG36950.0158.5989430
AGTCGCC68500.0113.3576429
TCGACAG201650.0102.6515228
GCGACTG2167550.092.7288628
AACGGAC52300.090.35480529
TCCCAGC328050.085.5507529
GCCTTCC150150.079.1526230
ATACCGC174600.075.7312929
GAGGTAT38700.073.379129
TCAGGGA277400.065.9921428
TTCGACA310600.058.59029827
TAACGGA41800.057.93079428
CGCGACT2281900.051.2397427
GGCGCCA321150.051.16161330
ACGGACA98600.050.54730