FastQCFastQC Report
Tue 28 Feb 2017
fastq1_norrna_nosnrna_notrna.fq

Summary

[OK]Basic Statistics

MeasureValue
Filenamefastq1_norrna_nosnrna_notrna.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147611610
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGAGTCGACCCAACGCGAAGATCGGAAGAGCACACGTCTGAAC9422410.6383244515793846Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGAGATCGGAAGAGCACACGTCT6798720.46058165749970476Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGAAGATCGGAAGAGCACAC5879910.39833655360848647No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACTC5453360.3694397750962814Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAGCTTGGGCTGCAGGTCGACCCGTGCAGATCGGAAGAGCACACGTCTGAA4767650.3229861120002688Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACATATACTAAAATTGGAACGATACAGAGAAAGATCGGAAGAGCACACGTC4090230.27709405784544994No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTCC3445850.2334403100135552Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTAAAATTGGAACGATACAGAGAAAGATCGGAAGAGCACACGTCTGAACTC3263260.22107068678405445Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAATACAAGCTTGGGCTGCAGGTCGACAGATCGGAAGAGCACACGTCTGAA3050200.2066368627779346Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATACTAAAATTGGAACGATACAGAGAAAGATCGGAAGAGCACACGTCTGAA2878980.1950375041637985Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCT2763890.18724069197538054Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGCTTGGGCTGCAGGTCGACCCGAGATCGGAAGAGCACACGTCTGAACTC2242990.15195213980797312Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TACTAAAATTGGAACGATACAGAGAAAGATCGGAAGAGCACACGTCTGAAC1970760.1335098235159145Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACTAAAATTGGAACGATACAGAGAAAGATCGGAAGAGCACACGTCTGAACT1913570.12963546702051418Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CACATATACTAAAATTGGAACGATACAGAGAAAGATCGGAAGAGCACACGT1818600.12320169124908265No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGA1738700.1177888378834158Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGGAGTCGAGCTCAACCCGCAACGCGAAGATCGGAAGAGCACACGTCTGA1619130.10968852653256746Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAAAATTGGAACGATACAGAGAAAGATCGGAAGAGCACACGTCTGAACTCC1497430.10144391758886717Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTACA2411950.042.4875371
CACTCGC1239000.041.753921
GGCTAGC603750.041.130055
TGTACAC2667700.040.9882242
GCTAGCT614850.040.555156
TCGCTTA440300.040.4425618
GAATACA2189700.040.2919241
GGGGTAT523500.039.931786
CTCGCTT456950.039.34307517
TACACGG3421550.038.9723134
GTACACG3468750.038.5712133
AATACAA2300800.038.4579622
TAGGGGT546750.038.2427944
GTCTAGG550800.038.1389161
AGGGGTA550650.037.9377565
GGGTATG554450.037.881267
TACAAGC2338450.037.7935564
ATACAAG2359300.037.4834143
GGTACAC398350.037.3424452
TCTAGGG562400.037.3316962