FastQCFastQC Report
Mon 3 Apr 2017
fastq1_norrna_nosnrna_notrna.fq

Summary

[OK]Basic Statistics

MeasureValue
Filenamefastq1_norrna_nosnrna_notrna.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences353336111
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGG23432400.6631759186368584Illumina Small RNA Adapter 2 (100% over 21bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGTTGGAATTCTCGGGTGCCAAG21958290.6214561522697011No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCTGGAATTCTCGGGTGCCAAGGAA14839170.41997320788986664RNA PCR Primer, Index 1 (100% over 23bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGTGGAATTCTCGGGTGCCAA13453920.38076832741276195No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAAG10405870.2945034395309796No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGGAATTCTCGGGTGCCAAGG8666460.24527524162397313Illumina Small RNA Adapter 2 (100% over 21bp)
GAATACAAGCTTGGGCTGCAGGTCGACTGGAATTCTCGGGTGCCAAGGAAC8516040.2410181052793667RNA PCR Primer, Index 1 (100% over 24bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAG6447940.18248743333228115No Hit
GGTCGCTGGTTCGTTTCCGGCTCGAAGGTGGAATTCTCGGGTGCCAAGGAA5340230.15113739676610635RNA PCR Primer, Index 1 (100% over 23bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCTGGAATTCTCGGGTGCCAAGGA5161480.14607847427176782RNA PCR Primer, Index 1 (100% over 22bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGCTGGAATTCTCGGGTGCCA4169610.11800690249856743No Hit
TAATCCACCTATAACTTCTCTGTTAACCCATGGAATTCTCGGGTGCCAAGG4110610.11633710430463191Illumina Small RNA Adapter 2 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATACA10640000.043.215811
AATACAA10812250.042.91332
ATACAAG10868850.042.657923
TACAAGC11272550.042.2190324
ACCCGTT3052300.041.80000326
CCGCCGA8733800.041.6224751
CCCGTTG3139600.040.63184727
GCCGTGA5368950.040.5060161
GGTCGAC11309500.040.14425321
GCTTGGG11891700.039.953369
CAAGCTT11908450.039.8753936
AGGTCGA11559800.039.7549320
CAGGTCG11745050.039.33055519
TGCAGGT11858250.039.1109417
CTTGGGC12214700.038.9040410
TTGGGCT12247200.038.72274811
ACAAGCT12328500.038.6978385
GGGCTGC12250650.038.4622613
GGCTGCA12300500.038.1473114
GTCGACC10584400.038.12743822