sORFs.org: repository of small ORFs identified by ribosome profiling

This section provides a summary on the acquisition and processing of the data. For more detailed information, feel free to consult the available literature. Use the navigation bar on the left to navigate trough the different steps.

9. BLASTp

BLASTp9 (Basic Local Alignment Search Tool protein) searches for AA-sequence similarity between the sorfs and the Non-redundant (NR) protein sequences database (NCBI). Sequence similarity with other proteins can provide additional information about the sorf. For intance 5'-UTR located sorfs predicted to have high sequence similarity with a protein isoform can indicate a false positive sORF. Additionailly sequence similarity can identify "PRIDE hits" that possibly originate from an other protein.